26Feb. 2017

Workshop at University of Medicine 1 at Yangon, Myanmar -Learning ways of Immunohistochemistry- 

Assoc. Prof. Ichiro Sakata 


Based on the agreement for partnership between Saitama University and University of Medicine 1 (UM1), a workshop titled “Principle and practice of immunohistochemistry” was held at UM1 at Yangon, Myanmar on January 5-6 2017. This event was held with support from Saitama University Lab-to-Lab program. Professor Ohmar from UM1 and Professor Mya Mya Thwin assisted with arrangements for this workshop, and eight PhD students from all five medical universities (UM1, University of Medicine 2 – UM2, University of Mandalay, University of Magway and University of Taunggyi) in Myanmar participated. In addition, Dr. Cho Cho Aung (UM 1) and Dr. Nyein Nyein Aye (UM 2) were assistant teachers at this workshop. Both academics stayed in Saitama University for one month in 2016 to learn immunohistochemistry using the Lab-to-Lab program.


On day one, the workshop began with greetings from Professors Ohmar and Sakai. Next, Professor Sakai gave a lecture on the basic principles and practical applications of immunohistochemistry. After lunch, Associate Professor Sakata explained the experiment details and schedule, and taught participants some tips. The immunohistochemistry experiment was performed under the supervision of Professor Sakai, Associate Professor Sakata, and Ms. Sakaida (a Master’s student from Saitama University). In this workshop, mice brains and rat pituitaries were stained with GnRH and LHb antibodies, respectively.


On day two, participants incubated the tissues with secondary antibodies and ABC complex, and the process of color detection was performed. Myanmar students performed the experiments well, and the expected results were obtained. Students were active during the lecture and practical training and asked many questions. After observing the stained slides, we had comments such as “it was a great experience and this two day experiment will help advance Myanmar research activities in the future.” We also discussed future collaborative research between Saitama University, UM1, and UM2.

  • Opening remarks from Professor Sakai
  • Associate Professor Sakata explained the immunohistochemistry procedure
  • The immunohistochemistry experiment at UM1 laboratory
  • All the workshop participants
  • Observation of stained slides with Professor Sakai